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mutagénesis en sitio dirigido
Mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule. The most common method involves use of a chemically synthesized oligonucleotide mutant which can hybridize with the DNA target molecule. The resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.
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- Branche/domæne: Agriculture
- Category: General agriculture
- Company: USDA
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